pcDNA3.1-3*Flag-HIF1A-WT Plasmid 

Catalog No. PVTB00599-2a

Vector Backbone pcDNA3.1(+)-3*Flag

Backbone manufacturer Invitrogen

Vector type Mammalian Expression

Backbone size w/o insert 5428bp

Cloning site 5 EcoR I

Site destroyed during cloning NO

Cloning site 3 Xho I

Bacterial resistance Ampicillin

Growth strain Top10

Growth temperature 37 Centigrade

High or low copy High Copy

Selectable markers Neomycin

Background Mammalian Expression vector of Homo sapiens hypoxia inducible factor 1 alpha subunit with N terminal 3xFlag tag

Gene Information

Alternative Gene Name HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA

Insert Gene name HIF1A-WT

Insert size 2481bp

Accessions NM_001530.3

Tag 3*Flag(N-term)

Species Homo sapiens (human)

Gene ID 3091

pcDNA3.1-3*Flag-HIF1A-WT Plasmid Sequence

        1 gactacaaag accatgacgg tgattataaa gatcacgaca tcgactacaa ggatgacgat 
       61 gacaagaagc ttggtaccga gctcggatcc gccaccatgg agggcgccgg cggcgcgaac 
      121 gacaagaaaa agataagttc tgaacgtcga aaagaaaagt ctcgagatgc agccagatct 
      181 cggcgaagta aagaatctga agttttttat gagcttgctc atcagttgcc acttccacat 
      241 aatgtgagtt cgcatcttga taaggcctct gtgatgaggc ttaccatcag ctatttgcgt 
      301 gtgaggaaac ttctggatgc tggtgatttg gatattgaag atgacatgaa agcacagatg 
      361 aattgctttt atttgaaagc cttggatggt tttgttatgg ttctcacaga tgatggtgac 
      421 atgatttaca tttctgataa tgtgaacaaa tacatgggat taactcagtt tgaactaact 
      481 ggacacagtg tgtttgattt tactcatcca tgtgaccatg aggaaatgag agaaatgctt 
      541 acacacagaa atggccttgt gaaaaagggt aaagaacaaa acacacagcg aagctttttt 
      601 ctcagaatga agtgtaccct aactagccga ggaagaacta tgaacataaa gtctgcaaca 
      661 tggaaggtat tgcactgcac aggccacatt cacgtatatg ataccaacag taaccaacct 
      721 cagtgtgggt ataagaaacc acctatgacc tgcttggtgc tgatttgtga acccattcct 
      781 cacccatcaa atattgaaat tcctttagat agcaagactt tcctcagtcg acacagcctg 
      841 gatatgaaat tttcttattg tgatgaaaga attaccgaat tgatgggata tgagccagaa 
      901 gaacttttag gccgctcaat ttatgaatat tatcatgctt tggactctga tcatctgacc 
      961 aaaactcatc atgatatgtt tactaaagga caagtcacca caggacagta caggatgctt 
     1021 gccaaaagag gtggatatgt ctgggttgaa actcaagcaa ctgtcatata taacaccaag 
     1081 aattctcaac cacagtgcat tgtatgtgtg aattacgttg tgagtggtat tattcagcac 
     1141 gacttgattt tctcccttca acaaacagaa tgtgtcctta aaccggttga atcttcagat 
     1201 atgaaaatga ctcagctatt caccaaagtt gaatcagaag atacaagtag cctctttgac 
     1261 aaacttaaga aggaacctga tgctttaact ttgctggccc cagccgctgg agacacaatc 
     1321 atatctttag attttggcag caacgacaca gaaactgatg accagcaact tgaggaagta 
     1381 ccattatata atgatgtaat gctcccctca cccaacgaaa aattacagaa tataaatttg 
     1441 gcaatgtctc cattacccac cgctgaaacg ccaaagccac ttcgaagtag tgctgaccct 
     1501 gcactcaatc aagaagttgc attaaaatta gaaccaaatc cagagtcact ggaactttct 
     1561 tttaccatgc cccagattca ggatcagaca cctagtcctt ccgatggaag cactagacaa 
     1621 agttcacctg agcctaatag tcccagtgaa tattgttttt atgtggatag tgatatggtc 
     1681 aatgaattca agttggaatt ggtagaaaaa ctttttgctg aagacacaga agcaaagaac 
     1741 ccattttcta ctcaggacac agatttagac ttggagatgt tagctcccta tatcccaatg 
     1801 gatgatgact tccagttacg ttccttcgat cagttgtcac cattagaaag cagttccgca 
     1861 agccctgaaa gcgcaagtcc tcaaagcaca gttacagtat tccagcagac tcaaatacaa 
     1921 gaacctactg ctaatgccac cactaccact gccaccactg atgaattaaa aacagtgaca 
     1981 aaagaccgta tggaagacat taaaatattg attgcatctc catctcctac ccacatacat 
     2041 aaagaaacta ctagtgccac atcatcacca tatagagata ctcaaagtcg gacagcctca 
     2101 ccaaacagag caggaaaagg agtcatagaa cagacagaaa aatctcatcc aagaagccct 
     2161 aacgtgttat ctgtcgcttt gagtcaaaga actacagttc ctgaggaaga actaaatcca 
     2221 aagatactag ctttgcagaa tgctcagaga aagcgaaaaa tggaacatga tggttcactt 
     2281 tttcaagcag taggaattgg aacattatta cagcagccag acgatcatgc agctactaca 
     2341 tcactttctt ggaaacgtgt aaaaggatgc aaatctagtg aacagaatgg aatggagcaa 
     2401 aagacaatta ttttaatacc ctctgattta gcatgtagac tgctggggca atcaatggat 
     2461 gaaagtggat taccacagct gaccagttat gattgtgaag ttaatgctcc tatacaaggc 
     2521 agcagaaacc tactgcaggg tgaagaatta ctcagagctt tggatcaagt taactgagcg 
     2581 gccgctcgag tctagagccc