Anti-RHOA antibody
Product Name Anti-RHOA antibody
Catalog No PAab07281
Packing 100ug
Form liquid
Alternative Name ARH12 antibody, ARHA antibody, RHO12
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695). (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
Immunogen ras homolog gene family, member A
Specificity Human, Mouse , Rat
Tested Application ELISA,WB,IHC,IF
Recommended dilution
IHC use
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using RHOA Antibody at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA bufferpH9).
WB use
human brain tissue were subjected to SDS PAGE followed by western blot with PAab07281(RHOA antibody) at dilution of 1:400
Protein Information
Gene ID387
Uniprot ID P61586
Calculated MW
location