Porcine ATP5G1 ELISA KIT
Product Name:Porcine ATP5G1 ELISA KIT
Packing:96T
Catalog No.:ELI-48992p
Gene Name:ATP5G1
Detect Range:0.78-50 ng/mL
Sensitivity:0.24ng/mL
Target Protein Name:Porcine ATP5G1
Alternative Name:ATP5G1,Porcine ATP5G1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Porcine ATP5G1 ELISA KIT allows for the in vitro quantitative determination of Porcine ATP5G1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Porcine ATP5G1 ELISA KIT has been pre-coated with an ATP5G1 antibody specific to Porcine ATP5G1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Porcine ATP5G1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Porcine ATP5G1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Porcine ATP5G1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!