Rat ACTG1 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  ACTG1

Protein Name  Actin, cytoplasmic 2

Alternative Name

Rat ACTG1 ELISA KIT , Rat ACT ELISA KIT ,Rat ACTG ELISA KIT ,Rat DFNA20 ELISA KIT ,Rat DFNA26 ELISA KIT ,Rat HEL-176 ELISA KIT ,Rat actin cytoplasmic 2 ELISA KIT ,Rat cytoskeletal gamma-actin ELISA KIT ,Rat epididymis luminal protein 176 ELISA KIT ,Rat actin gamma 1 ELISA KIT ,Rat actin gamma ELISA KIT

Intended use

Rat ACTG1 ELISA KIT allows for the in vitro quantitative determination of ACTG1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Rat ACTG1 ELISA KIT has been pre-coated with an ACTG1 antibody specific to ACTG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ACTG1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ACTG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACTG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20