Rat Serine/ threonine- protein kinase PLK1, PLK1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Plk1,serine/threonine-protein kinase PLK1; PLK; STPK13; PLK-1; cell cycle regulated protein kinase; polo (Drosophia)-like kinase; polo like kinase; serine/threonine-protein kinase 13; polo-like kinase 1

Search name

Rat Plk1 ELISA KIT ,Rat serine/threonine-protein kinase PLK1 ELISA KIT ,Rat PLK ELISA KIT ,Rat STPK13 ELISA KIT ,Rat PLK-1 ELISA KIT ,Rat cell cycle regulated protein kinase ELISA KIT ,Rat polo (Drosophia)-like kinase ELISA KIT ,Rat polo like kinase ELISA KIT ,Rat serine/threonine-protein kinase 13 ELISA KIT ,Rat polo-like kinase 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat serine/threonine-protein kinase plk1,Serine/threonine-protein kinase PLK1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/threonine-protein kinase PLK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serine/threonine-protein kinase PLK1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serine/threonine-protein kinase PLK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serine/threonine-protein kinase PLK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.